Two discussion groups that would be useful are the hometissueculture http://tech.groups.yahoo.com/group/hometissueculture/ and the Plant-tc List Serve
-- On 18 May 2012 17:34, Mega <masterstorm123@gmail.com> wrote:
Hey.
I'd like to try to make a tissue culture.
According to this http://www.biologie.uni-hamburg.de/b-online/d29/29a.htm
The first tissue cultures were done using nutirent agar and coconut milk (for plant hormones).
So what I intend to do:
Mix LB-agar powder with coconut milk. Then autoclave. BUT: Do the phyto-hormones survive autoclaving?
Or should I just put it in the microwave and hope all bacteria are killed??
--
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