We built the whole system including camera, filters, integrated gelbox etc at BIocurious. Email me off line and I can send you pics, designs for laser cutting etc. We never managed to put any documentation together, but the whole thing without the $90 camera is $100 or less. It works beautifully. We also designed gel cartridges that use a fraction of the reagents because we use channels instead of a full gel. Each lane uses 2ml. It also allows you to load dry without loading dye so it's fast and easier. You can experiment with it yourself to see if you like it. I can send you pics of the gels from the doc station. We use the Logitech c910 camera.
Cameron
On Tuesday, June 5, 2012 1:51:22 PM UTC-7, Ravi wrote:
-- On Tuesday, June 5, 2012 1:51:22 PM UTC-7, Ravi wrote:
Thanks all for advice/links. As I continue to work on this I will evaluate feasibility of kits, but I will definitely document. I know IOrodeo has kits but I think $300 is a little out of budget. Thanks to IOrodeo though for making there schematics completely available.Anyone have the Gelbox 2.0 laser cut schematics - I found the sketch up 3d files, but no laser files.Ravi
On Tuesday, June 5, 2012 10:18:52 AM UTC-4, Pieter wrote:The full instructions can be found on instructables:
- http://www.instructables.com/id/Gel-electrophoresis-system- mini/?ALLSTEPS
- http://www.instructables.com/id/UV-Transilluminator/? ALLSTEPS
Following these steps would probably also lead you to a fine gelbox: http://learn.genetics.utah.edu/content/labs/gel/build_ gel_box.pdf
The instructables are pretty nice, but it is sometimes hard to get all the components. Perhaps we should make a kit, as a supplement to the OpenPCR
On Tuesday, 5 June 2012 10:43:01 UTC+2, cory....@gmail.com wrote:I don't know anything about the Open Gelbox 2.0 but IORodeo has a gel
box which they give away the design files for (
http://www.iorodeo.com/content/mini-gel- )electrophoresis-system
including the spec sheet, Open SCAD files and laser-cutting files.
They also have python scripts in one of their Bitbucket repositories (
https://bitbucket.org/iorodeo/transilluminator_enclosure/src ) which
allow you to input different dimensions and it will generate the SCAD
files automatically. So if you want a larger or smaller box than the
default, you can easily make that happen.
As for the blue and amber filters for an LED illuminator, I had good
luck using gel filters (the colored things you see covering lights at
theater performances or movie sets). I got them from this guy on ebay
http://www.ebay.com/itm/390061292911?ssPageName=STRK: MEWAX:IT&_trksid=p3984.m1423. l2649
They have a huge selection of colors and they are very cheap ($6 for
4sq ft). If you want I can go through my notebook and find which
specific colors worked best.
No need to use an arduino to control the LEDs. If you have a DC power
supply, hook a few LEDs in series with a resistor and add a switch if
you want. Much cheaper.
Also, if you're interested in making a UV illuminator, IORodeo has
design files for that as well
http://www.iorodeo.com/content/transilluminator- enclosure
-cory
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