You can seal the whole works, and you won't lose any.
Two 2 liter bottles, connected by some PVC pipe, with one bottle in the ice bath, and the other with the butane and plant solutes. Dry ice and alcohol would work really well for the cold side. You can freeze the butane that way.
On Sat, Jan 5, 2013 at 3:45 PM, Ulysses1994XF04 <bensics@comcast.net> wrote:
Great info, thanks!
I guess it might be possible to do extractions of some plant/fungal/microbial cell products using chilled butane and recapturing it by distillation by encasing the condenser and receiving flask with ice and cold packs. If I lose a little, butane's cheap.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/6kSW6FbCZhUJ.
On Saturday, January 5, 2013 5:16:41 PM UTC-5, Ulysses1994XF04 wrote:I've been wanting to build a small, home biochemistry lab for a while but the biggest thing holding me back from pouring the time and money into it is that I just can't find any place that will sell the reagents I want to use to individuals (most companies that make laboratory-grade reagents will only sell to certified labs at universities and companies and such).--
I really want to do extractions of cellular products (enzymes, pigments, etc) of plants (grass clippings, dead flowers from my garden, etc) and fungi and bacteria I can just grow inside. For some of these, I need a good hydrophobic extraction solvent that's 1) as pure as possible and 2) has a low boiling point so I can easily evaporate/boil off (ideally, I would like to recapture it by distillation so I don't have to constantly buy more of it).
I can't find a single manufacturer that will sell chloroform, dichloromethane or small hydrocarbons like pentane and cyclopentane to individuals. What other hydrophobic solvents can I use?
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