Re: [DIYbio] Re: What are some good, hydrophobic solvents I can LEGALLY buy?

As Josiah suggested, methanol may work - it's often used isocratic with water in reverse phase HPLC ..... polar stuff comes off first, amphiphiles in the middle and hydrophobes last. Methanol boils at 68 Celsius. Perhaps ethanol will work instead of methanol is too hard to get ..... Everclear is 190 proof / 95 % ethanol and will boil about 78 C. Alcohols are commonly used to extract colors and flavors from plant material.

If by hydro phobic solvents you mean polar aprotic solvents - look at nail polish removers ... perhaps you can find bulks at salon supply stores?. Nail polish removers are typically one or more of ethyl acetate, acetone or acetonitrile. Any of Therese would extract your terpenes (saw more recent post).

>matt

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From: Ulysses1994XF04 <bensics@comcast.net>
To: "diybio@googlegroups.com" <diybio@googlegroups.com>
Sent: Sat, Jan 5, 2013 18:46:06 EST
Subject: [DIYbio] Re: What are some good, hydrophobic solvents I can LEGALLY buy?

Great info, thanks!

I guess it might be possible to do extractions of some plant/fungal/microbial cell products using chilled butane and recapturing it by distillation by encasing the condenser and receiving flask with ice and cold packs. If I lose a little, butane's cheap. 

On Saturday, January 5, 2013 5:16:41 PM UTC-5, Ulysses1994XF04 wrote:

I've been wanting to build a small, home biochemistry lab for a while but the biggest thing holding me back from pouring the time and money into it is that I just can't find any place that will sell the reagents I want to use to individuals (most companies that make laboratory-grade reagents will only sell to certified labs at universities and companies and such).

I really want to do extractions of cellular products (enzymes, pigments, etc) of plants (grass clippings, dead flowers from my garden, etc) and fungi and bacteria I can just grow inside. For some of these, I need a good hydrophobic extraction solvent that's 1) as pure as possible and 2) has a low boiling point so I can easily evaporate/boil off (ideally, I would like to recapture it by distillation so I don't have to constantly buy more of it).

I can't find a single manufacturer that will sell chloroform, dichloromethane or small hydrocarbons like pentane and cyclopentane to individuals. What other hydrophobic solvents can I use?

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