Re: [DIYbio] Re: Geneome Sequencing Costs

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Separate molecules in the same lane, or ligated end-on-end? Wouldn't
parallel reactions lead to multiple peaks simultaneously, leading to
difficulty establishing which molecule has which nucleotide?*

*Unless you did something really hackish with real-time-quantitative
PCR methods and added your molecules in discernably different molar
ratios, so you could try to correlate emission peak height with each
separate solution... O_o

On 02/27/2013 12:12 AM, Dietrich Dehlinger wrote:


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