Overnight culture generally has a range of 8-24 hours at least. Once the antibiotic has degraded then the issue becomes selecting for the plasmid. However, I have done preps on cultures that were > 24hours old and there was no issue.
On Tuesday, February 26, 2013 3:28:46 AM UTC-6, Mega wrote:
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Maybe, just maybe even as low as 30°C would be better...
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