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Once you're into long-chain PEG, the difference is often not such a big
deal. Try it with the same weight *or* molarity, ideally both, and see
if it works OK with either.
I'm sure there'll be guides on selecting chain-length for other
protocols, but I wouldn't be surprised if it's more of an art than a
science.
On 17/02/14 08:57, flight505 wrote:
> Hello,
>
> The lab i am working in only has peg 6000, the miller lab protocol
> for Gibson assembly master mix uses peg 8000
> can i adjust for the molecular weight deference and use peg 6000 instead ?
> does anyone have experience with this ?
>
> Best regards,
>
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Re: [DIYbio] Gibson assembly master mix
2:51 PM |
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