Re: [DIYbio] microfluidic chips to order

Sebastian, did you ever try using a laser-printed line for any experiments?

I ended up finding that the best program I had to use for printing the
crispest lines on a laser printer was MSPAINT or GIMP, using a black
and white (1 bit per pixel) BMP image. The number of colors doesn't
make it less crisp, but it takes WAY more space, and you need make
sure you're only using pure white or black.

I printed a bunch of test lines with all the different contrast
settings in the printer driver and found that InkScape applied
blurring on SVG and line-art, even if the linewidth was super-thin...
the BMP image editors printed lines that you could easily tell
individual pixels correlated to increasing line-width.

I did all that, and finally printed a test pattern (just some lines of
different thickness), but never actually went and exposed the
photo-resist! I'm too busy to do any lab work on this stuff for about
a month or so, but here's the image I made up:
http://nathanmccorkle.com/microfluidic_exposure_test_single_pixels.bmp

I deleted every-other pixel at the bottom of each line, so you can
visually tell how many pixels they are supposed to be when you're
zoomed in on an image program or when inspecting a printout under a
microscope.

On Fri, Feb 14, 2014 at 6:36 PM, Sebastian Cocioba <scocioba@gmail.com> wrote:
> I got the shrinking part down to a science so yet it would be flat enough
> for macrofluidic channels like 0.5mm+
>
> I use the vacuum plasma method to clean my PDMS and glass slide. It works
> well but again its more macro than micro. I've made gradient generators, pH
> sensors (two input laminar pH chromogenic marker flow thing), and some small
> mixing stuff. End goal was to use it for reliable protoplast isolation and
> culture. The shrinkydink white (clear sucks) is a bit temperamental but with
> a convection oven and small cookie sheet you can really get it to set flat.
> Just takes way longer than the 7min described by the mfg.
>
>
> Sebastian S. Cocioba
> CEO & Founder
> New York Botanics, LLC
> Plant Biotech R&D
> ________________________________
> From: Alexey Zaytsev
> Sent: 2/14/2014 9:27 PM
>
> To: diybio@googlegroups.com
> Subject: Re: [DIYbio] microfluidic chips to order
>
> Would the base surface be flat and smooth enough for a good adhesion to
> glass?
>
>
> On Sat, Feb 15, 2014 at 2:50 AM, Sebastian Cocioba <scocioba@gmail.com>
> wrote:
>>
>> I was actually speaking with an EE friend about just such a concept.
>> The smallest trace can go to about 7mil but it would be about 1-2mil
>> tall. He said using solder reflow could round off the channels. I made
>> a few shrinkydink chips with some success as long as the channel width
>> wasn't too small and only one layer. Dakota, if you want just send me a
>> hand drawn sketch and I'll make one for you in PDMS and pop it in the
>> mail for free. I have a ton of PDMS and shrinky paper so I don't mind.
>> I use DraftSight from dassault systems but AutoCAD is best for super
>> high res chips. Shoot me a pic of the chip u want. What's the worst
>> that can happen? :P
>>
>> Sebastian S. Cocioba
>> CEO & Founder
>> New York Botanics, LLC
>> Plant Biotech R&D From: Cathal Garvey
>> Sent: 2/14/2014 5:30 PM
>> To: diybio@googlegroups.com
>> Subject: Re: [DIYbio] microfluidic chips to order
>> No idea, I just recall seeing it discussed! :)
>> Looking at this handy adafruit ruler though, trace sizes can get pretty
>> small.. looks like 0.2mm on this ruler, and I expect they go lower with
>> some companies.
>>
>> On 14/02/14 22:04, Avery louie wrote:
>> > What trace size? Hit me up with the deets, I might know something.
>> >
>> > --A
>> > On Feb 14, 2014 4:38 PM, "Cathal Garvey" <cathalgarvey@cathalgarvey.me>
>> > wrote:
>> >
>> >> I've heard of people using circuitboard traces as a cast?
>> >>
>> >> On 14/02/14 21:27, Dakota Hamill wrote:
>> >>> Anyone know of any websites that would make a few chips to order, or
>> >>> give
>> >>> you a master mould? Sort of like 3D printing to order, but for
>> >>> microfluidics.
>> >>>
>> >>>
>> >>
>> >> http://www.fluigent.com/wp-content/uploads/2013/06/microfluidic-chip-micronit.jpg
>> >>>
>> >>> I'm looking for something exactly like that, except I want 3 channel
>> >> inputs
>> >>> at the top, not two, then the long pathway, with one exit.
>> >>>
>> >>> I'm pretty bad at using Sketchup so I've been trying to find design
>> >>> files
>> >>> someone else already made.
>> >>>
>> >>> Thanks
>> >>>
>> >>
>> >> --
>> >> Please help support my crowdfunding campaign, IndieBB: Currently at
>> >> 23.1% of funding goal, with 27 days left:
>> >> http://igg.me/at/yourfirstgmo/x/4252296
>> >> T: @onetruecathal, @IndieBBDNA
>> >> P: +3538763663185
>> >> W: http://indiebiotech.com
>> >>
>> >
>>
>> --
>> Please help support my crowdfunding campaign, IndieBB: Currently at
>> 23.1% of funding goal, with 27 days left:
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--
-Nathan

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