Re: [DIYbio] Re: Gene splicing equipment?

PCR cloning is the cheapest option most of the time ( you only need to perform PCRs and in some case blunt self-ligation, Gibson assembly is great ant time saving in case of multiple gene insertion or other extensive. Classic restriction enzyme method is very good gym :)

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> On 14 Feb 2014, at 22:37, Cathal Garvey <cathalgarvey@cathalgarvey.me> wrote:
>
> Alternatively, gibson assembly, but that's a whole other story. No
> ligases but a one-time cocktail of (3?) enzymes.
>
> I'm making a point of putting nice primer sites on IndieBB if it goes
> ahead so that it'll be gibson-friendly out of the box. :)
>
>
>> On 14/02/14 18:12, W. Estell wrote:
>> They will also need a DNA ligase.
>>
>> On Thursday, February 13, 2014 6:22:38 AM UTC-8, Mega [Andreas Stuermer]
>> wrote:
>>>
>>> I read that some people call editing DNA gene splicing.
>>>
>>> Basically what you need is plasmid DNA (
>>> http://www.indiegogo.com/projects/indiebb-your-first-gmo ) and
>>> restriction endonuclesases. And micropipette 10-100 uL plus 1-10 uL plus
>>> the tips plus tip boxes.
>>>
>>> And DNA you want to put into, and has the same restriction sites.
>
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> <0x988B9099.asc>

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