Re: [DIYbio] Re: IndieBB Campaign Survey; Help Appreciated

On Wed, Feb 19, 2014 at 8:30 AM, Mike Horwath <mikeh169@gmail.com> wrote:
> --What worries me is the unproven non-antibiotic selection. If it was easy,
> we'd already be seeing more of it...

I thought of this idea (self-selection, not specifically with colicin)
probably my first or second year of University, and the professors
didn't have any ideas on prior art and said it sounded unlike anything
they'd seen in the works.

Specifically my idea and question to them was, if we know the pathways
for say Kanamycin synthesis and know the Kanamycin resistance gene,
why not include them both on the same plasmid. I think the only answer
my Prof gave, if any, was that the operon for synthesis would be too
big... though that answer may have come from my own research or even
this very mailing list.

Now of course there are specifics of getting the drug outta the cell,
what's the mechanism of resistance (efflux pump, breakdown, some
endogenous enzyme requiring modification to not be affected, etc), but
I guess doing all that research has set up my mind to just love
Cathal's idea. He's got the evidence on mechanisms which seem like
they'd do the trick effectively, he's found/chosen genes/elements that
are small enough to fit on a 'normal sized' plasmid, he's got the
know-how to check for stupid hang-ups like frameshift errors, etc...
and on top of it all he's open-sourcing it, so during development
presumably he'd be asking for some community assessment/auditing
before dropping hundreds/thousands on synthesis (cause, you know,
unintentional frameshift errors SUCK).

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