[DIYbio] Re: large dna extraction

Just came across this accidentally... 



Am Montag, 24. Februar 2014 18:56:13 UTC+1 schrieb phillyj:
I never worked with large DNA (2-3 Mbase) before but I have a project
that required me to. I extracted the DNA from a gram positive bacteria
using invitrogen charge-switch magnetic beads kit. What I got on the
1% gel was a band around 15kb and a band way up in the wells. So it
might be that the 15kb band is the plasmid and the other band is gDNA.

Now, I'm running the sample thru a 0.5% agarose gel at 50V. I'm
planning on cutting out the plasmid and purifiying it with one of my
qiagen gel extraction kits. But, for the large gDNA, I don't have a
kit that can handle it. They usually max at 50kb.

How would you guys approach this? Ideas?

Thanks

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