You could try something like His tagging where you add a few histidine residues to the end of your protein (i.e introduced in your expression vector) and then isolate on a nickel column such as HiTrap from GE Healthcare, there's probably cheaper suppliers of alternatives.. These come as mini syringe versions for small preps rather than Acta scale protein prep systems.
On Friday, August 1, 2014 11:01:53 AM UTC+1, Mega [Andreas Stuermer] wrote:
-- On Friday, August 1, 2014 11:01:53 AM UTC+1, Mega [Andreas Stuermer] wrote:
Did a quick google research on it, no protocols found so far. How can I immagine dialysis step?
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