I've done a bit of AFM although it was in an institutional setting a few years ago and all the expensive kit was provided. It's a very intriguing technology along with other SPM methods. It's limitations seemed to be the depth of the image you can build up due to the physical limitations of the structure of the cantilever and at the time the newer SEMs for these methods seemed to give at least as good imaging plus deeper view of your sample's 'terrain'. The cantilevers that are used require microfabrication methods to make and are connected to a piezo material that feeds back signal depending on the feedback (can be physical but you can also look at electromagnetic feedback etc) from the sample.
-- I think the bottleneck in making these systems is being able to fabricate these probes.
Definitely an advanced project for hacking I'm really interested to see where it goes. It would be interesting to see if probes could be modified with biomolecular attachements and attempt to read feedback from interacting molecules or try to manipulate them, e.g pores that interact with DNA, enzymes or antibodies..
On Tuesday, July 29, 2014 10:09:33 PM UTC+1, Margret Storm wrote:
On Tuesday, July 29, 2014 10:09:33 PM UTC+1, Margret Storm wrote:
Neat!! Thanks for the info =)
On Sunday, July 27, 2014 8:50:41 PM UTC-4, Nathan McCorkle wrote:http://scholar.google.com/scholar?q=32.768+khz+crystal+ microscopy
http://research.sabanciuniv.edu/10163/1/Scanning_Hall_ Probe_Microscopy_(SHPM)_Using_ Quartz_Crystal_AFM_Feedback.
crystal is <$1 USD
http://www.newark.com/fox-electronics/nc38lf-327/tuning- fork-32-768khz-12-5pf-thru/dp/ 61T5432
similar application but some better images of the tips:
http://www.hindawi.com/journals/ijo/2012/591083/
http://diyhpl.us/~nmz787/pdf/Integrating_micro-_and_ nanoelectrodes_into_atomic_ force_microscopy_cantilevers_ using_focused_ion_beam_ techniques.pdf
http://diyhpl.us/~nmz787/pdf/Development_of_a_regeneration- type_neural_interface_A_ microtube_guide_for_axon_ growth_of_neuronal_cells_ fabricated_using_focused-ion- beam_chemical_vapor_ deposition.pdf
Also this:
http://hansmalab.physics.ucsb.edu/afmhistory.html
On Sun, Jul 27, 2014 at 1:21 PM, Margret Storm <i.am....@gmail.com> wrote:
> This is the paper they linked:
> http://www.uam.es/gruposinv/spmth/papers/2008_Science_332_ 413_Sugimoto_AFM.pdf
>
> It says: "We performed the AFM experiments (9) in
> dynamic mode under the frequency modulation
> detection scheme (10), keeping the cantilever
> oscillation amplitude constant. Commercial silicon
> cantilevers, which have very sharp tips at their
> free ends, were used to image the Sn/Si (111)
> […] R30° surface (11) by detecting the
> short-range chemical interaction force between
> the closest tip and surface atoms (9)."
>
> 9: http://arxiv.org/pdf/cond-mat/9706132.pdf
> 10: http://arxiv.org/pdf/cond-mat/0311522v1.pdf
> 11:http://journals.aps.org/pr/abstract/10.1103/PhysRev.141. (can't find a503
> full paper for this one, sorry)
>
>
> On Sunday, July 27, 2014 3:55:54 PM UTC-4, Nathan McCorkle wrote:
>>
>> Yes I saw that, but it doesn't seem to give a procedure used to produce
>> it, unless that is another paper (maybe referenced in that paper)?
>>
>> On Jul 27, 2014 10:54 AM, "Margret Storm" <i.am....@gmail.com> wrote:
>>>
>>> The third link's second image is of the atom used for manipulation.
>>>
>>> On Sunday, July 27, 2014 2:23:47 AM UTC-4, Nathan McCorkle wrote:
>>>>
>>>> I've been training to operate a Focused Ion Beam milling machine. It can
>>>> mill and deposit metal with a minimum spot size of about 10nanometers. I've
>>>> been making probes with a pipette puller then milling the ends with a
>>>> certain angle at a certain width along the probe tip.
>>>>
>>>> I think FIBs can make AFM probes... I didn't see a picture/diagram of
>>>> the tips they were using in those papers.
>>>>
>>>> On Jul 26, 2014 11:37 AM, "Margret Storm" <i.am....@gmail.com> wrote:
>>>>>
>>>>> These articles are very interesting:
>>>>> http://jb.asm.org/content/184/19/5205
>>>>> http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3246382/
>>>>>
>>>>> http://www.uam.es/gruposinv/spmth/highlights/2008_Science_ 322_413/highlight.html
>>>>>
>>>>> Has anyone here experimented with this technology themselves, or do you
>>>>> know anyone who has? What are your thoughts on the applications of the
>>>>> technology where it stands, or on how accessible it is? How viable would it
>>>>> be for modifying DNA/RNA sequences or proteins manually?
>>>>>
>>>>> --
>>>>> -- You received this message because you are subscribed to the Google
>>>>> Groups DIYbio group. To post to this group, send email to
>>>>> diy...@googlegroups.com. To unsubscribe from this group, send email to
>>>>> diybio+un...@googlegroups.com. For more options, visit this group at
>>>>> https://groups.google.com/d/forum/diybio?hl=en
>>>>> Learn more at www.diybio.org
>>>>> ---
>>>>> You received this message because you are subscribed to the Google
>>>>> Groups "DIYbio" group.
>>>>> To unsubscribe from this group and stop receiving emails from it, send
>>>>> an email to diybio+un...@googlegroups.com.
>>>>> To post to this group, send email to diy...@googlegroups.com.
>>>>> Visit this group at http://groups.google.com/group/diybio .
>>>>> To view this discussion on the web visit
>>>>> https://groups.google.com/d/msgid/diybio/06670b8b-23cd- .4fb0-a36d-969c579f2744% 40googlegroups.com
>>>>> For more options, visit https://groups.google.com/d/optout .
>>>
>>> --
>>> -- You received this message because you are subscribed to the Google
>>> Groups DIYbio group. To post to this group, send email to
>>> diy...@googlegroups.com. To unsubscribe from this group, send email to
>>> diybio+un...@googlegroups.com. For more options, visit this group at
>>> https://groups.google.com/d/forum/diybio?hl=en
>>> Learn more at www.diybio.org
>>> ---
>>> You received this message because you are subscribed to the Google Groups
>>> "DIYbio" group.
>>> To unsubscribe from this group and stop receiving emails from it, send an
>>> email to diybio+un...@googlegroups.com.
>>> To post to this group, send email to diy...@googlegroups.com.
>>> Visit this group at http://groups.google.com/group/diybio .
>>> To view this discussion on the web visit
>>> https://groups.google.com/d/msgid/diybio/72f69f5c-8e0b- .47ab-b2b0-2845b84b37cf% 40googlegroups.com
>>> For more options, visit https://groups.google.com/d/optout .
>
> --
> -- You received this message because you are subscribed to the Google Groups
> DIYbio group. To post to this group, send email to diy...@googlegroups.com.
> To unsubscribe from this group, send email to
> diybio+un...@googlegroups.com. For more options, visit this group at
> https://groups.google.com/d/forum/diybio?hl=en
> Learn more at www.diybio.org
> ---
> You received this message because you are subscribed to the Google Groups
> "DIYbio" group.
> To unsubscribe from this group and stop receiving emails from it, send an
> email to diybio+un...@googlegroups.com.
> To post to this group, send email to diy...@googlegroups.com.
> Visit this group at http://groups.google.com/group/diybio .
> To view this discussion on the web visit
> https://groups.google.com/d/msgid/diybio/95178c26-9be3- .4b56-8f02-8746827cea13% 40googlegroups.com
>
> For more options, visit https://groups.google.com/d/optout .
--
-Nathan
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/d8099210-1815-4124-a712-63a022ee814e%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.
0 comments:
Post a Comment