Re: [DIYbio] Re: Is it possible for a Homeworker to create a thermosensitive yoghurt?

Actually, I think this is a *much* easier proposition than the melamine
sensor (which never happened AFAIK). You can couple a gene system to
heat-shock promoters, and you're mostly there.

The complication is that the luciferin molecules are, I think, pretty
heat-labile. So, it might not work simply because the luciferin is being
pasteurised prior to oxidation by luciferase.

Also, some "heat" shock systems are actually just "shock" systems, and
may also activate under colder-than-ideal growth temperatures, so you
might find that what you really have is "glows when not at exactly 37C".

Covering for leaky expression would be important, but not that
important. As long as the luciferin/ase production is at the end of the
regulatory cascade and it scales nicely to input, some leaky expression
will be pretty invisible.

On 28/01/15 09:35, Jonathan Cline wrote:
>
> On Thursday, January 22, 2015 at 2:46:49 AM UTC-8, Lukas wrote:
>
> Hey Guys
>
> I want to create a thermosensitive yoghurt which reacts with
> bioluminescence under heat. I have the model of Meredith Patterson
> who has created a test for Melamin. I want to create the pudding for
> a school Project.
>
> Lg Lukas
>
> BTW if somebody has time : can you list the equipment for the
> experiment?
>
>
> It will take you years to develop this, if it ever works at all, though
> by the time you are done, you might have a Ph D. The equipment list is
> partially in the DIYbio FAQ http://openwetware.org/wiki/DIYbio/FAQ
>
> You probably want to look into Genomikon.
>
>
> ## Jonathan Cline
> ## jcline@ieee.org
> ## Mobile: +1-805-617-0223
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