Re: [DIYbio] Re: low cost positive pressure laminar flow HEPA filtered box

Could you mimic this though, by passing air through a HEPA and then
having it (inside the sterile space) pass through a lint-free,
pre-sterilised fabric sheet?

On 28/01/15 09:46, Patrik D'haeseleer wrote:
> On Tuesday, January 27, 2015 at 4:54:22 PM UTC-8, Otto Heringer wrote:
>
> There's also this well documented tutorial. The results looks nice.
> http://hackteria.org/wiki/DIY_Sterlisation_Hood
> <http://hackteria.org/wiki/DIY_Sterlisation_Hood>
>
>
> That one is definitely not a laminar flow hood though. For laminar flow,
> you want the entire air mass to move as one smooth sheet towards you (or
> form the ceiling to the floor, in vertical laminar flow).
> Because the Hackteria design only has one small cylindrical filter, the
> filtered air will flow out from there with lots of turbulence, and that
> turbulence can suck in contamination from the front of the hood. There's
> also no way they can match the recommended air volume of 100ft/min with
> a filter that small.
>
> The professional and DIY laminar flow hoods I've seen typically have a
> large rectangular HEPA filter that takes up the entire back surface of
> the hood, allowing the entire air volume to move towards you in one
> uniform mass. One of the links I posted above suggests how to check for
> laminar flow with a lighter: the flame of the lighter should be moving
> straight out away from the filter without any flickering whatsoever.
> That's a LOT of airflow!
>
> Patrik
>
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