Re: [DIYbio] Non-Platinum Electrophoresis Electrodes That Don't Degrade

Hi,

Just my two cents.

Have you considered to use the filament of electrical resistance of shower?

And the carbon brush used in electrical motors?

Or graphite of pencil?

Best Regards,
Markos



On 20-10-2015 03:19, PoeVA wrote:

Stainless is an attractive option, but be advised that it may give off [very] toxic chromate as the SS is electrochemically degrading.  Using SS for the anode could produce toxic (illegal to dump) chromate*.  I noticed a stern warning in an instructable I read a while ago about electrorefining or electrolyzing rust; it said to avoid stainless.  Both of those examples are from much larger amounts of metal electrolyzed over longer times, but even if you can't see the chromate, it will be there if your SS anode degrades.  It might seem fine (and low levels of chromate aren't optically visible), but I'm concerned that with long run times and buffer reuse it could accumulate; then if you have chronic exposure (ever had to run gel after gel after gel after...?  yeah.)  Last point, you don't want to be illegal or raise the hackles of the EPA and other regulatory agencies.

Best wishes,

Brian

* (as describe here).  

**(sorry, couldn't find it again before posting this, but I didn't have much time to look)

On Monday, May 27, 2013 at 9:12:25 PM UTC-4, Roger Stager wrote:

I have had good luck with stainless steel seizing wire from West Marine. It is $20 for 55 ft.  I often run 150v with TAE and no corrosion of the wire or discoloration of the buffer. 

Roger 

On May 26, 2013, at 2:29 PM, Josiah Zayner <josiah...@gmail.com> wrote:

I have been testing out a bunch of different metals and they either oxidize to death on the minutes timescale or in the case of stainless steel turn the buffer orange/brown.

Platinum wire is pretty expensive ~$1.00 cm^-1. Has anyone had luck with a cheaper metal at 100V or greater with TAE?

I have tried:

Nickel

Stainless Steel 430

Tinned Copper

Thinking of trying Titanium or Nickel Titanium (<= $4 meter^-1). Anyone ever tried these?

Thanks,

     Josiah Zayner

http://DoItOurselfScience.blogspot.com 

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diy...@googlegroups.com. To unsubscribe from this group, send email to diybio+un...@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+un...@googlegroups.com.
To post to this group, send email to diy...@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/d99d3d84-c707-4533-a8f5-64fbb2150ce8%40googlegroups.com?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.
 
 

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/e35cda6c-4f56-40c0-93f7-f03339dc36b9%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.