[DIYbio] Re: PCR trouble

Thanks

I did the PTC genotyping on myself and three others with great results each time, but it's a much smaller amplicon.

The plan is two part first I want to ligate it into the pDusk plasmid and try to get darkness activated bioluminescence, it'll be interesting to see how it reacts to its own light then. Depending on all the protein production and decay rates I may get glowing oscillator, or just something that glows dimly. Mostly this is practice for me to do PCR, restriction digest, PCR product purification, dephosphorylation, electrophoresis, and ligation, etc and all the design stuff along the way. That way I can do something challenging and make a bunch of mistakes and learn from them. Basically the labs I never had by not going to school.

I also plan to use those large fragments in a series of experiments to make a simplified and cheap protocol for sperm mediated gene transfer. Something that could be done in a lab space that costs less than $1000.

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