[DIYbio] Re: Receptor characterization

Andreas,

What specific species are you referring to? Generally people will clone the gene into an expression system to characterize it rather than try to purify the protein from tissue (very, very old school). Getting the cDNA for protein expression is often a challenge as you need the source RNA from the expressing tissue. Once you have the gene then you can focus on strategies to characterize it in what ever system you like.

Depending on your target species you might be able to get a Mammalian Gene Collection EST clone of your gene of interest.
http://genecollections.nci.nih.gov/MGC/

Harvard has some of the MGC EST with non-profit pricing (much lower than the commercial sources for the same clone).
https://plasmid.med.harvard.edu/PLASMID/Home.xhtml

Alternatively, you could send the sequence to a Gene Synthesis company if you have the money.

Cheers,
Scott

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