Re: [DIYbio] Internship Petshop.bio physical microbes store

On Apr 29, 2016 10:48 PM, "BraveScience" <bravescience@gmail.com> wrote:
>
> Not all people have the knowledge, time, equipment and skills to isolate mycelium through clonal reproduction.

That is what the internet/books and friends/mentors at for.

> Giving them the chance to receive and use ready-to-use material doesn't sound a crazy ripoff but a good opportunity to start correctly.
>
> Moreover have you ever tried to isolate tissue from mushroom cap?

Yes, you pressure-cook some agar, get a few pressure-cooked knives/razors/scalpels, then wipe a mushroom with alcohol and maybe dip it in a bleach + sterile water solution, rinse well with sterile water, then shave layers off the mushroom, for each cut alcohol dipping and flame sterilizing the cutting tool. If it it really dirty, switch cutting tools. When you get a nice clean chunk exposed, place onto agar. Leave in kitchen cabinet for a few days and you will see mycelium, after a few months untouched it is not uncommon to get a small micro/mini mushroom.

> Much easier to have some already growing mothefucking thick piece of mycelium.

If you can't handle what I described above, there's probably not too much more someone would be able to do with a plate of mycelium, other than look at it die or possibly fruit. But I guess maybe there's a demand quickly available generic mycelium... I've never seen it sold like that before anywhere, so hard to imagine a use case.

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