Re: [DIYbio] Re: DIY electroporator

I will test transformations on E.coli and Neurospora crassa.  I've had interest in a low cost electroporator for months.  I think the path to validation would be to show effectiveness with E. coli first (a much easier organism to transform).  Then transition to fungal cells which have much lower transformation efficiency.  If you have the hardware expertise to build a device with pre-programmed settings (say an E. coli and a fungal cells setting) I can do the lab work and prepare a data package for the product.  

-Will

On Saturday, April 30, 2016 at 9:54:35 AM UTC-4, John Griessen wrote:
On 04/29/2016 07:33 PM, William Beeson wrote:
> If anyone has ideas for how to get this to work well please post or share the ideas.  The amount of electrical engineering
> involved in this is beyond my skill level.

Will you commit to some testing?  Maybe 4 times on the same filamentous fungal cells?  Over weeks with little
test circuit boards in the mail?

You're only interested in having this yesterday, so as soon as it works for you, the testing stops?
If it doesn't get going in a week, you're out?

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