oh man, I hope I'm not as unfortunate! How does that happen though? did your fragment not include a stop codon?
On Wednesday, May 11, 2016 at 8:25:41 PM UTC-4, Scott wrote:
-- On Wednesday, May 11, 2016 at 8:25:41 PM UTC-4, Scott wrote:
Yes, pBluescript or pUC18 in DH5a with X-gal/IPTG will permit blue/white selection. If you clone (almost) anything (including a fragment with T7) into the MCS then you will inactivate the plasmid-born LacZa gene. I say almost because quite some time ago I PCR-amplified a small fragment and aimed to clone it into pUC13 in a specific direction relative to the polylinker but never got white colonies in that orientation. Turned out the fragment was one continuous ORF when inserted into the LacZa!
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/56ce28c2-0dde-42a7-bd45-84ce2cbbc470%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.
0 comments:
Post a Comment