[DIYbio] Re: pBLU and pBluescript

Yes, pBluescript or pUC18 in DH5a with X-gal/IPTG will permit blue/white selection. If you clone (almost) anything (including a fragment with T7) into the MCS then you will inactivate the plasmid-born LacZa gene. I say almost because quite some time ago I PCR-amplified a small fragment and aimed to clone it into pUC13 in a specific direction relative to the polylinker but never got white colonies in that orientation. Turned out the fragment was one continuous ORF when inserted into the LacZa!

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