Re: [DIYbio] E. coli with pVIB not glowing, why?

Closer to 30C, room temperature while the weather was mild.

On Sunday, July 30, 2017 at 6:48:07 PM UTC-6, Sebastian wrote:

Did you grow it at 37C or 30C? 37 deactivates the enzyme

On Sunday, February 26, 2017 at 12:35:16 PM UTC-5, Nathan McCorkle wrote:

On Feb 25, 2017 9:56 AM, "MC" <marky...@gmail.com> wrote:
>
> That's really interesting, I'm going to have to do some reading on recombination. Both my cultures were DH10B.

Oh, ok, I guess the-odin changed how the sell pVIB... their product page showed this week that they ship pVIB in BL21.

Hard to say what could've happened from my end, then.

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