[DIYbio] Re: stable transformation of chlorella vulgaris

thanks a lot, the transformation i am planing to do is based on using the flanking regions of nitrate reductase which will cause knockout of nitrate reductase and the selection method i will apply will be use of potassium chlorate. the knockout of nitrate reductase gens will protect the transformed cells against the toxic effect of chlorate. i was planing to used as a selection. method.

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