Re: [DIYbio] stable transformation of chlorella vulgaris

I can help what do you need? What's your location? What's the delivery molecule? 

On Wed, Jul 11, 2018, 5:48 AM najib abdellaoui <najibveto@gmail.com> wrote:
hello everyone 
i am currently working on a project for production of vaccine based on stable transformation of chlorella vulgaris. the plasmid size is around 6 Kb and i wanted to transformed into chlorella vulgaris using electroporation to get a stable expression of my protein of interest and also i wanna analyze the site of integration into genome.  we have bio-rad gene pulser II in our lab.
so can someone help me for the design of protocol for the transformation.
thanks in advance.

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