Re: [DIYbio] stable transformation of chlorella vulgaris

I can help what do you need? What's your location? What's the delivery molecule? 

On Wed, Jul 11, 2018, 5:48 AM najib abdellaoui <najibveto@gmail.com> wrote:

hello everyone 

i am currently working on a project for production of vaccine based on stable transformation of chlorella vulgaris. the plasmid size is around 6 Kb and i wanted to transformed into chlorella vulgaris using electroporation to get a stable expression of my protein of interest and also i wanna analyze the site of integration into genome.  we have bio-rad gene pulser II in our lab.

so can someone help me for the design of protocol for the transformation.

thanks in advance.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/b0614187-01f9-4217-9075-3651c203d2df%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.

To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAKjWJJzk_9HiUqBLWzh0Omb9Q32AoaWEovM_WKHksb-S5Norfw%40mail.gmail.com.

For more options, visit https://groups.google.com/d/optout.