Re: [DIYbio] Re: Paper electrophoresis... super available molecule separation?

Congrats on trying this and it working!

Here are some papers I just dug on regarding nucleic acids (mono and
poly-mers), there's a lot of data on pH charge-dependence effecting
migration (but basically you want the pH above 3 or 4), also saying it
took an hour for Uridine 3'-phosphate (charge of -1 between pH 2 and
5) to move 8cm at 20V/cm:
http://nathanmccorkle.com/pdf/paper_electrophoresis/

including:
DETERMINATION OF ADENINE NUCLEOTIDES BY PAPER ELECTROPHORESIS.pdf
Paper Electrophoresis of Nucleic Acid Components.pdf
Practical Applications of Paper Electrophoresis.pdf
Techniques for elution of nucleic acid components from paper.pdf
The Use and Limitations of Filter-paper Electrophoresis.pdf

On Wed, Jan 30, 2013 at 12:00 AM, Sebastian S. Cocioba
<scocioba@gmail.com> wrote:
> So I tried it again with loading dye without wrapping it in parafilm and it
> worked. The loading dye moved across the strip without smearing but the
> transit distance was no where near where it would have been had the medium
> been agarose.
>
> I put 5uL of concentrated loading dye near the end of the strip of coffee
> filter paper. I set the strip between two microscope slides and carefully
> laid the slide sandwich onto the chamber floor and filled it with standard
> TAE buffer. I ran the sandwich at 25v for one hour just to see if the dye
> would budge.
>
> The main issue I see is the lack of dispersion control in the initial
> loading and the staining of the strip post run with EtBr. Im going to get
> some white filter paper and try to stain the dna ladder post run once more.
> It would be very interesting if this works.
>
> Sebastian S Cocioba
> CEO & Founder
> New York Botanics, LLC
>
> Sent via Mobile E-Mail
>
> On Jan 30, 2013, at 1:06 AM, Patrik D'haeseleer <patrikd@gmail.com> wrote:
>
> Hey Cathal - do you have some Kombucha going right now? I'd love to see what
> happens if you take a nice thick "mother" SCOBY, cut some wells into it, and
> use it as an electrophoresis gel. :-). Food dyes electrophorese pretty well.
>
> Patrik
>
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--
-Nathan

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